BS ISO 3632-2:2010 pdf free.Spices – Saffron (Crocus sativus L.) Test methods.
BS ISO 3632-2 For each slide (6.5.2 to 6.5.4), take a test portion of the order of 0,001g to 0,002 g saffron in powder (see 10.3) or crushed filament (see 10.2) form.Preparation for observation in water Prepare two slides as follows.Deposit 50 μl of water on a slide. Using the tip of a scalpel or lancet needle, take the test portion (6.5.1), mix it with the water and wait for at least 5 min to ensure that the powder is adequately wet before covering with a cover slide.
Preparation for observation in an aqueous solution of sodium hydroxide, potassium hydroxide or chloral hydrate
Prepare two slides as indicated in 6.5.2, but replace water with the sodium hydroxide, potassium hydroxide or chloral hydrate aqueous solutions (6.3.2).
Wait for a few minutes for the medium to iluminate and observe for 10 min after adding the iluminating solution in order to avoid altering the cellular elements and ensuring they can be ientified.
This observation enables ilumination of the preparations by destroying tally or parially the major part of the cellular contents. The cellular elements are also made clearer and easier to observe, particularly the sclerous elements, vessels, fibres and epidermis.
Preparation for observation in aqueous iodine in iodide solution Prepare a slide as indicated in 6.5.2, but replace the water with iodine in iodide solution (6.3.1). NOTE This observation makes visible the starch grains which are stained blackish blue or blackish violet.
Observation, identification, and counting Place each slide, prepared according to 6.5.2 to 6.5.4, under the microscope (6.4.7). Set the magnification at 100 times. Identify and count the elements observed with a magnification of 400 times (see 6.7).
The anatomical structures and exogenous elements are identified and counted for each slide on an observation of 10 fields.
If the microscope used (6.4.7) is equipped with a device permiting observation in polarized light, one of the two slides prepared in 6.5.2 should be so observed.Figure 1 shows an example which summarizes all operations permitting counting.BS ISO 3632-2 pdf download.